Process Development Associate , Vectors
Company: Yoh, A Day & Zimmermann Company
Location: Somerville
Posted on: February 17, 2021
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Job Description:
Process Development Scientist ( Downstream, Bioprocess, Viral
Vectors, AAV) 305174
RESPONSIBILITIES:
Bioprocess;
Downstream Process Development, process optimization for
manufacturing viral vectors
Vector Engineering/ Viral Vectors. Support vector core Vector
Engineering processes, Process Development
Support vector core vector engineering processes, process
development, and process optimization
Process Development, process optimization, Bioprocess
Upstream/ Downstream Process Development, process optimization for
manufacturing viral vectors
Bioprocess development of AAV vectors focusing, upstream
cultivation and harvest conditions to optimize yield
Working with AAV viral particles, fermentation, plasmid recovery,
purification and formulation, sterilization, and harvesting process
steps
Apply research/ industry knowledge of Lentivirus (LV) and or Adeno
Associated Virus (AAV)
Cell Line maintenance, Cell Line development, Media development; as
cell are transfected with plasmids to generate viral vectors, which
are efficiently harvested
Creating and maintaining stable cell lines
Troubleshooting and optimizing stepwise.
Plasmid Preparation, Buffer, Media Preparation, Aseptic Processing,
growth & maintenance of cell lines.
Handling Bioreactors, reagent/media prep, sterile manufacturing,
upstream/downstream processing
Transfection, generate viral vectors, and efficient harvesting
Troubleshooting and optimizing processes stepwise.
Apply skills in cGMP and gene therapy, vector purification, process
scale-up, and sterile manufacturing
Bioprocess development of AAV vectors focusing, upstream
cultivation and harvest conditions to optimize yield
Managing suspension cultures, downstream purification recoveries,
and vector potency
Overall support of upstream process development for novel gene
therapy vector.
REQUIREMENTS:
Bachelor's (BS) or Master's (MS) in Molecular Biology, Virology,
Biology, Biochemistry or related
Minimum of a 4-8 yrs. + experience in the Biotech, Cell & Gene
Therapy (CGT) industry working with viral vectors
In depth knowledge of both upstream processing and or downstream
processing
Knowledge of protein purification, mammalian cell culture,
virology, viral validation, Preformulation
Formulation, ultracentrifugation, flocculation, scale-able
chromatography and tangential flow filtration (TFF).
Process Development (PD), manufacturing of viral vectors;
transfection, transcription, translation, AAV vector
in vivo genetic modification, Plasmid DNA Extraction
4-8 yrs. + experience handling bioreactors, media development, and
process development/ bioprocess refinement
4-8 yrs. + experience in protein and gene therapy, vector
purification, process scale-up, and sterile manufacturing
Upstream/Downstream and the equipment and tools used in that
process including; bioreactors, Vi-CELL, S.U.M.'s, sterile tubing
welders, filters, balances, microscopes, pipettes, CellSTACK's,
HYPERstack's Clipsters, DeltaV, BSC's, pH Meters, Osmometers,
Autoclaves etc.
Sterile manufacturing, cGMP, cleanroom, Upstream / Downstream
Process Development and GMP compliance.
UPSTREAM - 20L-200L bioreactors, cell expansion, transient
transfection, viral production and clarification
DOWNSTREAM -Recovery and purification of viral vectors and removal
of contaminants. Nuclease digestion, Purification, Sterile
Filtration and Fill
ANALYTICS - PAT, feed rates, pH control, Potency measurement,
Bioburden, Mycoplasma, Endotoxin
cGMP, sterility, fermentation, mammalian cell culture, bioprocess
optimization, purification, including clarification by filtration/
centrifugation; chromatography, ion exchange, sizing, multi-modal
and other resins
Tangential Flow Filtration (TFF) or Ultracentrifugation/
Diafiltration (UF/DF), Size Exclusion Chromatography.
PLEASE:
Share your updated resume using E-MAIL ADDRESS,
philip.cooke@yoh.com
CALL 860-405-7181 (anytime up until 7pm) to discuss
#ZipSPG
#LI-PC
Yoh, a Day & Zimmermann company, is an Equal Opportunity Employer.
All qualified applicants will receive consideration for employment
without regard to race, color, religion, sex, sexual orientation,
gender identity, national origin, disability, or status as a
protected veteran. Visit
https://www.yoh.com/applicants-with-disabilities to contact us if
you are an individual with a disability and require accommodation
in the application process.PandoLogic.Category: Science, Keywords:
Biochemist
Keywords: Yoh, A Day & Zimmermann Company, Somerville , Process Development Associate , Vectors, Other , Somerville, Massachusetts
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