Process Development Associate , Vectors

Company: Yoh, A Day & Zimmermann Company
Location: Somerville
Posted on: February 23, 2021

Job Description:

Process Development Scientist ( Downstream, Bioprocess, Viral Vectors, AAV) 305174

RESPONSIBILITIES:

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• Bioprocess;- Downstream Process Development, process optimization for manufacturing viral vectors
  
• Vector Engineering/ Viral Vectors. Support vector core Vector Engineering processes, Process Development
  
• Support vector core vector engineering processes, process development, and process optimization
  
• Process Development, process optimization, Bioprocess
  
• Upstream/ Downstream Process Development, process optimization for manufacturing viral vectors
  
• Bioprocess development of AAV vectors focusing, upstream cultivation and harvest conditions to optimize yield
  
• Working with AAV viral particles, fermentation, plasmid recovery, purification and formulation, sterilization, and harvesting process steps
  
• Apply research/ industry knowledge of Lentivirus (LV) and or Adeno Associated Virus (AAV)
  
• Cell Line maintenance, Cell Line development, Media development; as cell are transfected with plasmids to generate viral vectors, which are efficiently harvested
  
• Creating and maintaining stable cell lines
  
• Troubleshooting and optimizing stepwise.
  
• Plasmid Preparation, Buffer, Media Preparation, Aseptic Processing, growth & maintenance of cell lines.
  
• Handling Bioreactors, reagent/media prep, sterile manufacturing, upstream/downstream processing
  
• Transfection, generate viral vectors, and efficient harvesting
  
• Troubleshooting and optimizing processes stepwise.
  
• Apply skills in cGMP and gene therapy, vector purification, process scale-up, and sterile manufacturing
  
• Bioprocess development of AAV vectors focusing, upstream cultivation and harvest conditions to optimize yield
  
• Managing suspension cultures, downstream purification recoveries, and vector potency
  
• Overall support of upstream process development for novel gene therapy vector.
  
  
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  REQUIREMENTS:
  
  
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  • Bachelor's (BS) or Master's (MS) in Molecular Biology, Virology, Biology, Biochemistry or related
    
  • Minimum of a 4-8 yrs. + experience in the Biotech, Cell & Gene Therapy (CGT) industry working with viral vectors
    
  • In depth knowledge of both upstream processing and or downstream processing
    
  • Knowledge of protein purification, mammalian cell culture, virology, viral validation, Preformulation
    
  • Formulation, ultracentrifugation, flocculation, scale-able chromatography and tangential flow filtration (TFF).
    
  • Process Development (PD), manufacturing of viral vectors; transfection, transcription, translation, AAV vector
    
  • in vivo genetic modification, Plasmid DNA Extraction
    
  • 4-8 yrs. + experience handling bioreactors, media development, and process development/ bioprocess refinement
    
  • 4-8 yrs. + experience in protein and gene therapy, vector purification, process scale-up, and sterile manufacturing
    
  • Upstream/Downstream and the equipment and tools used in that process including; bioreactors, Vi-CELL, S.U.M.'s, sterile tubing welders, filters, balances, microscopes, pipettes, CellSTACK's, HYPERstack's Clipsters, DeltaV, BSC's, pH Meters, Osmometers, Autoclaves etc.
    
  • Sterile manufacturing, cGMP, cleanroom, Upstream / Downstream Process Development and GMP compliance.
    
  • UPSTREAM - 20L-200L bioreactors, cell expansion, transient transfection, viral production and clarification
    
  • DOWNSTREAM -Recovery and purification of viral vectors and removal of contaminants. Nuclease digestion, Purification, Sterile Filtration and Fill
    
  • ANALYTICS - PAT, feed rates, pH control, Potency measurement, Bioburden, Mycoplasma, Endotoxin
    
  • cGMP, sterility, fermentation, mammalian cell culture, bioprocess optimization, purification, including clarification by filtration/ centrifugation; chromatography, ion exchange, sizing, multi-modal and other resins
    
  • Tangential Flow Filtration (TFF) or Ultracentrifugation/ Diafiltration (UF/DF), Size Exclusion Chromatography.
    
    
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    PLEASE:
    
    
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    • Share your updated resume using E-MAIL ADDRESS,
      
    • CALL 860-405-7181 (anytime up until 7pm) to discuss
      
      
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      Yoh, a Day & Zimmermann company, is an Equal Opportunity Employer. All qualified applicants will receive consideration for employment without regard to race, color, religion, sex, sexual orientation, gender identity, national origin, disability, or status as a protected veteran. Visit https://www.yoh.com/applicants-with-disabilities to contact us if you are an individual with a disability and require accommodation in the application process.PandoLogic. Keywords: Biochemist, Location: Somerville, MA - 02145

Keywords: Yoh, A Day & Zimmermann Company, Somerville , Process Development Associate , Vectors, Other , Somerville, Massachusetts